@groovy.util.logging.Log class VariantInjector extends java.lang.Object
Injects a variant into a FASTQ read based on recognising the sequence corresponding to the reference sequence for the location of the variant within the read.
| Type | Name and description |
|---|---|
double |
alleleFraction |
boolean |
debugMode |
int |
nReplaced |
int |
padding |
gngs.FASTA |
reference |
java.lang.String |
referenceBases |
java.lang.String |
referenceBasesRC |
java.lang.String |
replacementBases |
java.lang.String |
replacementBasesRC |
int |
total |
gngs.Variant |
variant |
int |
variantOffset |
| Constructor and description |
|---|
VariantInjector
(gngs.FASTA reference, gngs.Variant variant, int variantOffset) |
| Type Params | Return Type | Name and description |
|---|---|---|
|
void |
computeReplacementBases()Computes the corresponding sequence after a reference sequence is mutated by a variant which must be a SNV, insertion or deletion |
|
void |
inject(gngs.FASTQRead r1, gngs.FASTQRead r2)Injects the variant for this VariantInjector into the given reads, and adjusts the base quality string accordingly. |
|
boolean |
inject(gngs.FASTQRead r, java.lang.String refBases, java.lang.String repBases, boolean random) |
|
void |
renameRead(gngs.FASTQRead r1, gngs.FASTQRead r2) |
| Methods inherited from class | Name |
|---|---|
class java.lang.Object |
java.lang.Object#wait(long), java.lang.Object#wait(long, int), java.lang.Object#wait(), java.lang.Object#equals(java.lang.Object), java.lang.Object#toString(), java.lang.Object#hashCode(), java.lang.Object#getClass(), java.lang.Object#notify(), java.lang.Object#notifyAll() |
Computes the corresponding sequence after a reference sequence is mutated by a variant which must be a SNV, insertion or deletion
Injects the variant for this VariantInjector into the given reads, and adjusts the base quality string accordingly.
r1 - read 1r2 - read 2Groovy Documentation